Investigations on the infestation of ticks and the prevalence of Borrelia burgdorferi and Babesia divergens in cattle in Bavaria

By Edelhofer, R. and Just, F. T. and Lengauer, H. and Pfister, K., Berliner Und Munchener Tierarztliche Wochenschrift, 2006
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Description
During the grazing period 2002 319 cattle from 31 farms located in 6 districts of southern Bavaria were examined for the presence of ticks in 4- to 5-week intervals, and 287 serum samples were tested for the presence of antibodies against Borrelia burgdorferi and Babesia divergens. Ticks were detected in all 31 farms with a mean prevalence of 69%. 3218 out of 3453 collected ticks were Nodes ricinus; 139 nymphs, 19 larvae and 77 damaged adult specimens could only be determined to the Genus level (Ixodes). The seasonal pattern revealed the highest frequencies of ticks in May/June and September. The intensity of tick infestation of positive animals was generally low. 76.5% of parasitized cattle had 1-6 ticks per day of investigation. Individual cattle showed up to 250 ticks per day. The percentage of infested animals in each herd varied within the period between 0-100 %. The examination of serum samples by immunfluorescence technique (IFAT) revealed positive anti-Borrelia antibody titers (>= 1:64) for 45.6% of the animals. The within-farm seroprevalence of borreliosis ranged from 20 to 100% in 27 of the 31 farms. A significant correlation could be detected between the number of ticks/cattle and the anti-Borrelia burgdorferi IgG-titer. By contrast, there was no significant correlation between the age of the animals and anti-Borrelia serum titers. For comparative reasons, 64 IFAT-positive serum samples were tested by Westernblot techniques for the presence of antibodies cross-reacting with Borrelia gorinii antigen. These analyses revealed that 69% of the samples reacted positively, 28% were unclear and 3 % were negative. Examinations of the 287 serum samples for the presence of anti-Babesia divergens antibodies revealed one positive animal with a titer of 1:16.
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